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Registros recuperados: 10 | |
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Ky, Chin-long; Demmer, Jonathan; Blay, Carole; Lo, Cedrik. |
Pinctada margaritifera is an economically important marine bivalve species for cultured pearl production in French Polynesian aquaculture. In order to evaluate the influence of donor oyster age on pearl quality traits, experiments were conducted over 6 years using both grafts and surgreffe operations. At harvest, 6 pearl quality traits were recorded and compared: surface defects, luster, grade, darkness level, and visual color. Analyzing the quality traits of pearls harvested in the initial graft process and those of pearls obtained from surgreffe experiments allowed a comparison of the influence of pearl sac cells originating from the initial mantle graft, which aged together with their recipient oysters. The results demonstrated a significant decrease... |
Tipo: Text |
Palavras-chave: Pearl oyster age; Pinctada margaritifera; Surgreffe; Pearl grade; Pearl colour. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00283/39472/37953.pdf |
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Blay, Carole; Planes, Serge; Ky, Chin-long. |
Grafting mantle tissue of a donor pearl oyster into the gonad of a recipient oyster results in the formation of a chimera, the pearl sac. The phenotypic variations of this chimera are hypothesized to be the result of interactions between the donor and recipient genomes. In this study, the heritability of phenotypic variation and its association with gene expression were investigated for the first time during P. margaritifera pearl production. Genetic variance was evaluated at different levels, 1) before the graft operation (expression in graft tissue), 2) after grafting (pearl sac tissue expression in chimera) and 3) on the product of the graft (pearl phenotype traits) based on controlled bi-parental crosses and the F1 generation. Donor related genetic... |
Tipo: Text |
Palavras-chave: Gene expression; Heritability; Pearl oyster; Phenotype; Pinctada margaritifera. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00433/54505/55876.pdf |
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Blay, Carole; Planes, Serge; Ky, Chin-long. |
Nucleated pearls are produced by molluscs of the Pinctada genus through the biomineralisation activity of the pearl sac tissue within the recipient oyster. The pearl sac originates from graft tissue taken from the donor oyster mantle and its functioning is crucial in determining key factors that impact pearl quality surface characteristics. The specific role of related gene regulation during gem biogenesis was unknown, so we analysed the expression profiles of eight genes encoding nacreous (PIF, MSI60, PERL1) or prismatic (SHEM5, PRISM, ASP, SHEM9) shell matrix proteins or both (CALC1) in the pearl sac (N = 211) of Pinctada margaritifera during pearl biogenesis. The pearls and pearl sacs analysed were from a uniform experimental graft with sequential... |
Tipo: Text |
Palavras-chave: Biomineralisation; Relative gene expression; Pearl sac; Biomarkers; Pearl quality; Pinctada margaritifera. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00435/54622/56022.pdf |
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Blay, Carole; Planes, Serge; Ky, Chin-long. |
Grafting associates two distinct genotypes, each of which maintains its own genetic identity throughout the life of the grafted organism. Grafting technology is well documented in the plant kingdom, but much less so in animals. The pearl oyster, Pinctada margaritifera, produces valuable pearls as a result of the biomineralisation process of a mantle graft from a donor inserted together with a nucleus into the gonad of a recipient oyster. To explore the respective roles of donor and recipient in pearl formation, a uniform experimental graft was designed using donor and recipient oysters monitored for their growth traits. At the same time, phenotypic parameters corresponding to pearl size and quality traits were recorded. Phenotypic interaction analysis... |
Tipo: Text |
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Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00387/49809/50382.pdf |
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Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Vanaa, Vincent; Lo, Cedrik; Cabral, Philippe. |
Individual Pinctada margaritifera molluscs were collected from the Takapoto atoll (Tuamotu Archipelago, French Polynesia) and used to produce ten first generation full-sib families in a hatchery system, following artificial breeding protocols. After three years of culture, these progenies were transferred to Rangiroa atoll (Tuamotu Archipelago, French Polynesia) and tested for their potential as graft donors. A large-scale grafting experiment of 1500 grafts was conducted, in which a single professional grafter used ten individual donor oysters from each of the ten families, grafting 15 recipient oysters from each donor. The recipient oysters were all obtained from wild spat collection in Ahe (Tuamotu Archipelago, French Polynesia). After 18 months of... |
Tipo: Text |
Palavras-chave: Cultured pearl oyster; Pearl quality; Progeny effects; Heritability; Genetic selection; Pinctada margaritifera; French Polynesia. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00152/26310/24380.pdf |
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Blay, Carole; Haffray, Pierrick; Bugeon, Jérôme; D’ambrosio, Jonathan; Dechamp, Nicolas; Collewet, Guylaine; Enez, Florian; Petit, Vincent; Cousin, Xavier; Corraze, Geneviève; Phocas, Florence; Dupont-nivet, Mathilde. |
One of the top priorities of the aquaculture industry is the genetic improvement of economically important traits in fish, such as those related to processing and quality. However, the accuracy of genetic evaluations has been hindered by a lack of data on such traits from a sufficiently large population of animals. The objectives of this study were thus threefold: (i) to estimate genetic parameters of growth-, yield-, and quality-related traits in rainbow trout (Oncorhynchus mykiss) using three different phenotyping technologies [invasive and non-invasive: microwave-based, digital image analysis, and magnetic resonance imaging (MRI)], (ii) to detect quantitative trait loci (QTLs) associated with these traits, and (iii) to identify candidate genes present... |
Tipo: Text |
Palavras-chave: Aquaculture; Fat content; Flesh colour; Magnetic resonance imaging; Fatmeter; Computer vision; Genetic correlations; QTL. |
Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00682/79410/81958.pdf |
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Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Lo, Cédrik; Cabral, Philippe. |
The top aquaculture species in French Polynesia is Pinctada margaritifera, a mollusc grown for the production of a unique gem: the black pearl. One of the challenges facing the pearl farming industry is to "produce less but better pearls" through genetic improvement. An experimental hatchery system was used to generate full-sib families to be tested for their potential as donor "oysters". A large-scale grafting experiments was done and seven cultured pearl quality traits: grade, surface defects, lustre, darkness level, visual colour categories, circles and shape categories were recorded. Our results revealed, for the first time, significant phenotypic relationships between these quality traits. The grade A cultured pearl class had the largest proportion of... |
Tipo: Text |
Palavras-chave: Pearl "oyster"; Pinctada margaritifera; Donor "oyster"; Cultured pearl quality; Pearl grade; Pearl colour; Pearl shape; Selective breeding. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00190/30130/28592.pdf |
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Ky, Chin-long; Blay, Carole; Aiho, Vaite; Cabral, Philippe; Le Moullac, Gilles; Lo, Cedrik. |
In French Polynesia, the aquaculture of P. margaritifera is carried out in numerous grow-out sites, located over three archipelagos (Gambier, Society and Tuamotu). To evaluate the impact of macro-geographical effects of these growing sites on pearl quality traits, five hatcheries produced families were used as homogeneous donor oysters in an experimental graft. The molluscs were then reared in two commercial locations: Tahaa island (Society) and Rangiroa atoll (Tuamotu). At harvest, eight pearl quality traits were recorded and compared: surface defects, lustre, grade, circles, shape categories, darkness level, body and secondary colour and visual colour categories. Overall inter-site comparison revealed that: 1) all traits were affected by grow-out... |
Tipo: Text |
Palavras-chave: Pinctada margaritifera; Cultured pearl quality; Grow-out location; Environment. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00276/38687/37186.pdf |
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Blay, Carole; Planes, Serge; Ky, Chin-long. |
Ageing is defined as the progressive decline in tissue and organ functions over time. This study aims to evaluate the ageing effect on cultured pearl quality phenotypes (including size and quality traits) in the graft-recipient animal model: Pinctada margaritifera. For this, eight uniform grafting experiments were designed using two hatchery-produced pearl oyster families as donors, which were followed through time, between 7 and 30 months in age. For each age category, 20 donors were studied for each culture site giving a total of 2400 grafted oysters. Several phenotypic measurements were made: 1) donor family growth performance from shell size records, 2) pearl size and corresponding quality traits, and 3) expression of some genes related to... |
Tipo: Text |
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Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00444/55593/57220.pdf |
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Ky, Chin-long; Blay, Carole; Broustal, Floriane; Sham Koua, Manaarii; Planes, Serge. |
Molluscs display a vast range of shell colours both between and within species. However, only a few species show colour variation in their soft tissues. In French Polynesia, the pearl oyster Pinctada margaritifera has three tissue morphotypes: the black wild-type and two rare mutations: white albino and orange mantle. Phenotypic transmission is known to occur from these phenotypes when they are used as graft donors for pearl production, leading to multicoloured and white pearls from black and albino mantle grafts, respectively. The present study furthers this knowledge by examining the phenotypic association between the orange mantle tissue morphotype and hard tissues: shells and cultured pearls. Based on a large experimental graft, shell colour... |
Tipo: Text |
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Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00486/59760/62878.pdf |
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Registros recuperados: 10 | |
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